Kaidao Paste (Ling) _TCM treatment of cancer_Jiaozuo Zhonghai TCM Tumor Hospital

ESOPHAGEAL CANCER

95% were squamous cell carcinoma.
China is one of the regions with high incidence of esophageal cancer in the world, with an average of 150000 deaths every year.
The typical symptom is progressive dysphagia. First, it is difficult to swallow dry food, then it is semi-liquid food, and finally it is impossible to swallow water.
Prognosis: The overall five-year survival rate after surgery or radical radiotherapy and chemotherapy is 20%, among which the expected survival rate of patients with early esophageal cancer after five years of radical treatment is usually more than 90%, and patients without any treatment generally die within one year.
Henan, Hebei and Shanxi are the top three in the incidence of esophageal cancer in China. Among them, Linzhou, Anyang, Huixian and other places in Henan Province are China, and also the regions with the highest incidence rate and mortality of esophageal cancer in the world, with the incidence rate as high as 478/100000.
The total population of the high incidence area of esophageal cancer in Henan Province is more than 20 million, accounting for 21% of the total population in Henan Province.

Indications of Kaidao Paste

Open the esophagus, detumescence and anticancer, and soften the tumor.
It is applicable to esophageal cancer and advanced cardiac cancer, resulting in inability to eat, drink, water, rice, and vomiting.
Generally, the esophagus can be opened after 3-7 days of medication, and people can drink water to eat.

Cytoproliferative toxicity test of Kaidao ointment

Take the esophageal squamous cell carcinoma Eca109 cell line and treat the measured data after 24 hours and 48 hours according to the design concentration

Enlightened-24h-ECA109

Description: After sterilization of Kaidaoling stock solution. Dilute twice with PBS to reduce viscosity. The cells were treated with 0.1 times, 0.05 times, 0.025 times, 0.0125 times and 0.00625 times the final concentration of the original solution for 24 hours, and then the cell proliferation-toxicity was measured with CCK-8. The action curve is shown in the figure. IC500.030 times the concentration of the stock solution. (The culture media of all cells are centrifuged to remove the solid insoluble matter. Because the high concentration has the background color of the drug, each concentration is separately set with a control hole)

Enlightened-48h-ECA109

Note: After sterilization of Kaidaoling stock solution, dilute it with PBS once to reduce the viscosity. The final concentration of use is 0.1 times, 0.05 times, 0.025 times, 0.0125 times, 0.00625 times of the stock solution. After 48h treatment of cells, use CCK-8 to measure the cell proliferation-toxicity. The action curve is shown in the figure. IC500.028 times the concentration of the stock solution. (The culture media of all cells are centrifuged to remove the solid insoluble matter. Because the high concentration has the background color of the drug, each concentration is separately set with a control hole)

Feeding tube squamous cell carcinoma Te-1 cell line, the data were measured after 24 hours and 48 hours treatment according to the design concentration

Enlightened-24H-TE1

Note: After sterilization of Kaidaoling stock solution, dilute it with PBS twice to reduce its viscosity. The cells were treated with 0.1 times, 0.05 times, 0.025 times, 0.0125 times and 0.00625 times the final concentration of the original solution for 24 hours, and then the cell proliferation-toxicity was measured with CCK-8. The action curve is shown in the figure. IC500.023 times the concentration of the stock solution. (The culture media of all cells are centrifuged to remove the solid insoluble matter. Because the high concentration has the background color of the drug, each concentration is separately set with a control hole)

Enlightened-48H-TE1

Note: After sterilization of Kaidaoling stock solution, dilute it with PBS twice to reduce its viscosity. The cells were treated with 0.1 times, 0.05 times, 0.025 times, 0.0125 times and 0.00625 times the final concentration of the original solution for 48 hours, and then the cell proliferation-toxicity was measured with CCK-8. The action curve is shown in the figure. IC50=0.014 times the concentration of the stock solution. (The culture media of all cells are centrifuged to remove the solid insoluble matter. Because the high concentration has the background color of the drug, each concentration is separately set with a control hole)

Take the KSY450 cell line of esophageal squamous cell carcinoma and treat the data after 24 hours and 48 hours according to the design concentration

Enlightened-24H-KSY450

Note: After sterilization of Kaidaoling stock solution, dilute it with PBS twice to reduce its viscosity. The cells were treated with 0.1 times, 0.05 times, 0.025 times, 0.0125 times and 0.00625 times the final concentration of the original solution for 24 hours, and then the cell proliferation-toxicity was measured with CCK-8. The action curve is shown in the figure. IC500.024 times the concentration of the stock solution. (The culture media of all cells are centrifuged to remove the solid insoluble matter. Because the high concentration has the background color of the drug, each concentration is separately set with a control hole)

Enlightened-48H-KSY450

Note: After sterilization of Kaidaoling stock solution, dilute it with PBS once to reduce the viscosity. The final concentration of use is 0.1 times, 0.05 times, 0.025 times, 0.0125 times, 0.00625 times of the stock solution. After 48h treatment of cells, use CCK-8 to measure the cell proliferation-toxicity. The action curve is shown in the figure. IC50=0.015 times the concentration of the stock solution. (The culture media of all cells are centrifuged to remove the solid insoluble matter. Because the high concentration has the background color of the drug, each concentration is separately set with a control hole)

Feeding tube squamous cell carcinoma KYSE510 cell line, the measured data were processed according to the design concentration for 24 hours and 48 hours respectively

Enlightened-24H-SKY510c

Note: After sterilization of Kaidaoling stock solution, dilute it with PBS twice to reduce its viscosity. The cells were treated with 0.1 times, 0.05 times, 0.025 times, 0.0125 times and 0.00625 times the final concentration of the original solution for 24 hours, and then the cell proliferation-toxicity was measured with CCK-8. The action curve is shown in the figure. IC50=0.027 times the concentration of the original solution. (The culture media of all cells are centrifuged to remove the solid insoluble matter. Because the high concentration has the background color of the drug, each concentration is separately set with a control hole)

Enlightened-48H-SKY510

Note: After sterilization of Kaidaoling stock solution, dilute it with PBS once to reduce the viscosity. The final concentration of use is 0.1 times, 0.05 times, 0.025 times, 0.0125 times, 0.00625 times of the stock solution. After 48h treatment of cells, use CCK-8 to measure the cell proliferation-toxicity. The action curve is shown in the figure. IC50=0.021 times the concentration of the original solution. (The culture media of all cells are centrifuged to remove the solid insoluble matter. Because the high concentration has the background color of the drug, each concentration is separately set with a control hole)

From the results of cell proliferation-toxicity experiment, we can see that Kaidao ointment can inhibit cell proliferation and induce cell death in a concentration-dependent manner. The mode of cell death includes active cell death - programmed cell death, cell apoptosis and passive cell death, that is, cell necrosis.
In addition, autophagy-mediated cell death is another main way for drugs to kill tumor cells. By means of protein immunoblotting, the marker proteins LC3-b, Bcl-2, Bax and the indicator protein PARP of cell apoptosis were detected. The results showed that Kaidao ointment could indeed cause cancer cell apoptosis and autophagy, and did not harm normal cells.

Leadership care

Liu Yanhua, Counselor of the State Council and former Vice Minister of the Ministry of Science and Technology
Professor Liu Baoyan, former executive vice president of the Chinese Academy of Traditional Chinese Medicine and academician of the Eurasian Academy of Sciences
Professor Chen Shilin, Director of Institute of Medicine, Chinese Academy of Traditional Chinese Medicine and academician of Eurasian Academy of Sciences

Professor Liu Yanhuai, President of Beijing Dongfang Lingdun Technology Co., Ltd. and expert group member of China National Intellectual Property Administration, led the delegation to visit

He Ruiping, Chairman of Guangdong Fengcheng Group
Gao Xuhua, Chairman of Guangdong Dapeng Pharmaceutical Group
Chairman of Boya Education Group
Visit our hospital for inspection and drug cooperation promotion

Clinical case analysis

Clinical case analysis(1)

On June 10, 2019, due to chest discomfort and swallowing obstruction, the pathological examination of the Second People's Hospital of Fuyang City, Zhejiang Province confirmed that "esophageal squamous cell carcinoma grade II, esophageal space occupying, 1.0cmx1.0cm mass in the esophagus".

He came to our hospital before July 16, 2019. When he saw the doctor, he did not eat water and rice, and he vomited when he ate it. After taking Kaidao ointment, he could eat noodles for five days. After two months of traditional Chinese medicine treatment, he was again diagnosed with electronic gastroscopy, and the results of pathological examination showed "chronic inflammation of esophageal mucosa".

Clinical case analysis(2)

Wu Zixiang, when seeing a doctor, it was difficult to get into the water and rice for more than a month. He could drink milk on the third day and eat noodles on the fifth day after using our Kaidao cream.

Pathological examination results before treatment: esophageal cancer.

Pathological examination results after treatment: reflux esophagitis.

Clinical case analysis(3)

Zhang Yu, male, 72-year-old, from Baotou, Inner Mongolia, with esophageal and cardiac adenocarcinoma.

On April 15, 2019, his son and daughter-in-law were treated on behalf of him without any treatment. He choked when eating, swallowed poorly, had white and thick coating on his tongue, and was weak all over. After four courses of medication in our hospital, he ate smoothly,

Check everything is normal!

Clinical case analysis(4)

Cao Linhua, a 65-year-old female, lives in Dalaidian Village, Jiuzhou Road, Qibin District, Hebi City. In August 2017, esophageal squamous cell carcinoma was found, and 20 times of radiotherapy had no obvious effect. She came to our hospital before October 2, 2017, and was unable to swallow water at the time of treatment. By taking our traditional Chinese medicine preparation "Kaidao Ointment", she was able to drink water and eat for a week, and two courses of traditional Chinese medicine treatment, and everything was normal.

Three years later, the re-examination showed chronic esophagitis with stable condition.

Clinical case analysis(5)

Liu Guozheng, male, 75 years old, lives in Zhangwu Village, Xitao Town, Wuzhi County, Jiaozuo City, suffering from cardiac cancer.

He came to our hospital for treatment on August 16, 2018. He coughed, swallowed poorly, burped, and did not think about food.

After treatment in our hospital, we were able to eat normally and in good spirits. The reexamination showed that our condition was obviously improved and tended to be normal.